The dual band fluorescence filter sets contain dual bandpass excitation and emission (barrier) filters, which are incorporated into a single element capable of selectively isolating fluorescence emission from two fluorophores simultaneously. Each of the filter sets is designed for optimal performance with a specific fluorochrome pair, although they are equally effective with alternate pairs of fluorescent probes that have similar absorption and emission spectral profiles. Utilizing precise wavelength band selection, with steep bandpass transitions between reflection and transmission regions, the multiple excitation and emission signals are separated with minimal crossover interference.
Filter Type | Dual Band Fluorescence Filter Sets | ||
Coating Type | Hard Coated | ||
Part Number | 30012 | ||
Individual Filter | Excitation Filter | Emission Filter | Dichroic Mirror |
475nm/23nm | 525nm/23nm | 500nm | |
CWL/FWHM | 560nm/19nm | 606nm/34nm | 580nm |
Angle of Incidence | 0±5° | 0±5° | 45° |
Transmission | >85% | >85% | >98% |
Size(mounted) | Ø25mm x 5mm | Ø25mm x 3.5mm | Ø25.2mm x 35.6mm x 1.05mm |
Wavelength Tolerance | ±3nm | ||
Blocking | OD>5 | ||
Scratch-Dig | 60-40 | ||
Substrate | Fused Silica, B270 | ||
Compatible Microscopes | Most common Nikon, Olympus, and Zeiss Microscopes | ||
Compatible Fluorophore | DPH, AMCA-X, MEQ, AMC, AMCA,DMACA,4-Methylumbelliferone,6-Amino-Quinolin,7-Hydroxy-4-Methylcoumarin,ß-methlyumbelliferone,ABQ,Alexa Fluor® 350,Aminocoumarin,Bis-aminophenyl-oxadizole ,Bisbenzamide (Hoechst 33258),Blue GFP,CellTracker™ Blue CMAC,Coumarine maleimide (CPM). | ||
Fluorescence Image Gallery | Click here or contact us to get |
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